视频搜索
热门关键字:
当天更新0部 总数量1473

ABCDEFGHIJKLMNOPQRSTUVWXYZ

热门排行榜

推荐视频

  • Microfab..

    主讲人:请看下面内容介绍

    类型:Cellular Biology

    时长:未知

    时间:2008

    推荐:

  • Title Ce..

    主讲人:请看下面内容介绍

    类型:Cellular Biology

    时长:未知

    时间:2007

    推荐:

  • Micro-sc..

    主讲人:请看下面内容介绍

    类型:Cellular Biology

    时长:未知

    时间:2007

    推荐:

  • Patterni..

    主讲人:请看下面内容介绍

    类型:Cellular Biology

    时长:未知

    时间:2007

    推荐:

当前位置:首页  »  实验视频  »  Cellular Biology  »  Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2

  • Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2


    时长:未知

    类型:Cellular Biology

    时间:2009

    国家:美国

    推荐星级:

    播放次数:加载中

    包包:20免费充值包包

    .

    主讲人:请看下面内容介绍  

播放地址1: FLV文件(说明: 无需安装任何插件,即可快速播放 )

内容介绍

    Judy Yen, Ron Golan, Kathleen RubinsWhitehead Institute for Biomedical Research, Massachusetts Institute of Technology The family Poxviridae consists of large double-stranded DNA containing viruses that replicate exclusively in the cytoplasm of infected cells. Members of the orthopox genus include variola, the causative agent of human small pox, monkeypox, and vaccinia (VAC), the prototypic member of the virus family. Within the relatively large (~ 200 kb) vaccinia genome, three classes of genes are encoded: early, intermediate, and late. While all three classes are transcribed by virally-encoded RNA polymerases, each class serves a different function in the life cycle of the virus. Poxviruses utilize multiple strategies for modulation of the host cellular environment during infection. In order to understand regulation of both host and virus gene expression, we have utilized genome-wide approaches to analyze transcript abundance from both virus and host cells. Here, we demonstrate time course infections of HeLa cells with Vaccinia virus and sampling RNA at several time points post-infection. Both host and viral total RNA is isolated and amplified for hybridization to microarrays for analysis of gene expression.

ICP证( 吉ICP备09002134号) | 管理员信箱:stemcell8@qq.com |  干细胞之家论坛

页面执行时间: 3.4160秒 54次数据查询