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当前位置:首页  »  实验视频  »  Cellular Biology  »  Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

  • Fluorescence in situ hybridization (FISH) Protocol in Human Sperm


    时长:未知

    类型:Cellular Biology

    时间:2009

    国家:美国

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    播放次数:加载中

    包包:20免费充值包包

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    主讲人:请看下面内容介绍  

播放地址1: FLV文件(说明: 无需安装任何插件,即可快速播放 )

内容介绍

    Zaida Sarrate, Ester AntonUnitat de Biologia Cel·lular (Facultat de Biociències), Universitat Autònoma de Barcelona Aneuploidies are the most frequent chromosomal abnormalities in humans. Most of these abnormalities result from meiotic errors during the gametogenic process in the parents. In human males, these errors can lead to the production of spermatozoa with numerical chromosome abnormalities which represent an increased risk of transmitting these anomalies to the offspring. For this reason, the technique of fluorescence in situ hybridization (FISH) on sperm nuclei has become a protocol widely incorporated in the context of clinical diagnosis. This practice provides an estimate of the frequencies of numerical chromosome abnormalities in the gametes of the patients that seek for genetic reproductive advice. To date, the chromosomes most frequently included in sperm FISH analysis are chromosomes X, Y, 13, 18 and 21. This video-article describes, step by step, how to process and fix a human semen sample, how to decondense and denature the sperm chromatin, how to proceed to obtain sperm FISH preparations, and how to visualize the results at the microscope. Special remarks of the most relevant steps are given to achieve the best results.

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